KMID : 0364820180540030208
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Korean Journal of Microbiology 2018 Volume.54 No. 3 p.208 ~ p.213
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Characterization of anti-inflammatory effect of soybean septapeptide and its molecular mechanism
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Lewis Kevin M.
Sattler Steven A. Kang Chul-Hee Wu Hong-Min Kim Sang-Geon Kim Han-Bok
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Abstract
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Activation of nuclear factor kappa B (NF¥êB) leads to theinflammatory process. During this NF¥êB-dependent inflammationprocess, inducible nitric oxide synthase (iNOS) are expressedin the inflammatory cells. Our previous data indicated that aspecific septapeptide (GVAWWMY) from the soybean extractfermented by Bacillus licheniformis B1 inhibited iNOS mRNAexpression and NO production in cultured macrophage cells. Ourfurther experiments revealed that treatment of same septapeptideresulted in inhibition of LPS-induced NF¥êB activation byreversing degradation of I¥êB¥á, an inhibitory protein for NF¥êB.
The molecular docking indicated that the septapeptide binds toI¥êB kinase ¥â (IKK¥â), and thus it can inhibit phosphorylation ofI¥êB¥á. Supporting this, the binding site for the septapeptide hasthe highest affinity (-8.7 kcal/mol) and the site was located atthe kinase domain (KD) of IKK¥â, which can significantlyaffect the kinase activity of IKK¥â.
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KEYWORD
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ermented soybean, I¥êB¥á, IKK¥â, molecular docking, NF¥êB, septapeptide
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